Hengyuan share the latest countermeasures for common problems in ELISA experiments - Database & Sql Blog Articles

Hengyuan has shared the common problems and solutions of elisa kits. Now, after the hard work of our technicians, we have developed the latest countermeasures for common problems of ELISA kits. Let's take a look at it. :
1. The absorbance value of the negative sample is lower than the positive absorbance value. The reason may be the phenomenon of jumping holes or the insufficient mixing of the reagents in the wells of the microplate. The solution is to pay attention to the method of operation, pay attention to the method of washing, after adding the reagent, the enzyme plate can be gently shaken on the table for 30s, and the liquid is mixed.
Second, the high value of the absorbance is what may be caused by the temperature of the incubation is too high, the reaction time is too long. The corresponding solution is to adjust the temperature of the incubation environment. If the temperature in the room cannot be adjusted, shorten the color development time and control the reaction time.
3. Samples should be thoroughly mixed before and after dilution. All reagents should be shaken before loading to ensure uniformity of the test.
The ELISA reaction is a highly sensitive reaction. If the serum is not diluted, a strong non-specific reaction will inevitably occur and a false positive will occur. Therefore, the kit for detecting serum antibodies abroad requires that the serum be diluted to an appropriate multiple to reduce the non-specific reaction, so that the specific antigen-antibody reaction is fully reflected. The dilution factor of the sample of the general product is determined by a large number of tests to ensure the sensitivity and specificity of the test. However, some users fail to follow the instructions carefully. For example, some products should be diluted with 10μl samples, and individual users should take 5μl or even 1μl samples for dilution, because the sample is inevitably contaminated with the sample and the accuracy of the micropipette. Not enough, so the sample dilution factor is not accurate, and the test results are problematic.
Can these help you? You are welcome to pay attention to the latest developments of our company. If you have any questions, please feel free to contact us.