Why do dual-wavelengths must be used when reading the microplate reader? -Huaqiang Electronic Network

Why do dual-wavelengths must be used when reading the microplate reader? This problem is often mentioned by customers in the detection of elisa kits. To understand correctly, you need to follow the instructions. In this chapter, Shanghai Jinma Bio must use dual wavelength for case reading when reading the microplate reader. Welcome to learn!
First of all, the microplate reader must be preheated for 10-15 minutes before use, which makes the test results more stable. Secondly, the ELISA test uses two wavelengths to measure the absorbance, which can eliminate the measurement interference (concentration of the specimen, interference color, etc.) at the time of single wavelength detection. Generally, the maximum wavelength is used as the reference wavelength. At the reference wavelength, the absorbance of the detection object is the smallest, and the difference between the absorption wavelength of the detection wavelength and the reference wavelength can eliminate non-specific absorption; therefore, the dual-wavelength measurement can eliminate fingerprints and impurities to the utmost extent. And the error caused by the opaque material on the microplate reader reading to ensure the accuracy of the experimental data.
We carefully select imported antibody pairs, standards, chromogenic reagents and ELISA plates to achieve optimal performance. It adopts advanced patented technology and is assembled and produced in strict accordance with the standard production plan. Batch import of raw materials and self-contained special formulation thinner ensure high-quality product performance while taking into account cost. Welcome to inquire Elisa kit.